Nutrient SamplingSampling refers to the process of selecting units or portions of a larger group that will be studied in order to answer questions about the larger group. The units can be people, water samples, ice cores, or any other appropriate object. Participants will explore the meaning of sampling and how it impacts experimental design and explore factors that define and limit sampling in the variety of projects visited during the expedition. They will consider how results from the chosen samples are used to describe the bigger target of a project's study.

    Early sea conditions prevented the science team from collected large seawater samples using the Nisken bottles. Once we were far enough offshore, however, the science team was able to start collecting small volume samples using the ship's flow-through system. This system collects sea water and pumps it into the wet lab near the aft (back) section of the main deck. While we waited for better weather to deploy larger equipment, I spent time with William (Will) Abbott from the University of South Florida (USF) who is in charge of nutrient sampling for this research cruise. Will is using a flow injection analyzer (FIA) to determine the concentration levels of dissolved inorganic nutrients in ocean water samples.

    Flow Injection Analyzer (FIA)
    Will Abbott points to the various hoses, pumps and reactants used in the flow injection analyzer (FIA). This automated system can detect nutrient levels in the water. On this research cruise, the FIA will be used to identify concentrations of nitrate, nitrite, ammonium, silicate and phosphate.

    Will Abbott's Lab Notebook
    Working in a lab requires meticulous notes. All of the scientists on this research cruise have one or more lab notebooks to record data, make notations, complete calculations, etc. Will Abbott makes notes about the sample number, ship's location and time for future reference.

    The FIA works by pumping seawater samples past different reagents that will react with specific nutrients. These chemical reactions will allow the computer to analyze the samples and determine the concentrations of the desired nutrients. For the purposes of this cruise, the desired nutrients include nitrate, nitrite, ammonium, silicate and phosphate.

    Reagent set-up
    Will Abbott begins connecting chemical reagents to the flow injection analyzer (FIA). These reagents will interact with certain nutrients in the sea water to analyze concentrations.

    Flow injection analyzer computer readout
    The computer monitor shows the data collected by the flow injection analyzer. The graphs along the bottom of the screen indicates the concentrations of nutrients in the sea water sample.

    Will begins sampling water as soon as possible in order to monitor the conditions of the water as we travel through the Drake passage. In talking with Will and Dr. Kristen Buck of USF, I also learned that Will's data will help determine when we have reached a specific area of the Southern Ocean where specific types of diatoms are likely to be found. DiatomsDiatoms are one of the most common types of phytoplankton. Most diatoms are unicellular, although they can exist as colonies in the shape of filaments or ribbons. Diatom communities are a popular tool for monitoring environmental conditions, past and present, and are commonly used in studies of water quality. have a outer "shell" called a silica frustule. As the name implies, these frustules are essentially made using the silica from the ocean water to build this outer "shell", similar to a cell wall in plants. Finding the areas of high silicate allow Dr. Buck and the other scientists to decide where to sample in the hopes of collecting specific types of phytoplankton.

    Troubleshooting

    Will spent most of the day prepping the FIA machine, remixing some reagents and sampling water from the flow-through system. I was given the opportunity to put on some goggles, gloves and a lab coat and act as assistant for the day. I collected water every 30 minutes and helped Will trouble shoot a problem with the FIA. Ok - I watched while Will did the troubleshooting, but he said he enjoyed the company.

    Silicate channel
    Notice the blue coloration in the tubes at the top right of the image? This coloration change is not expected in this silicate analysis channel. This coloration tells the controller that something is wrong with the set-up. Chemicals can degrade faster than expected or react differently due to chemicals. Each one of these factors is taken into consideration when troubleshooting problems.

    While Will continued to work on the FIA nutrient sampling, I stepped outside to help with the first trace metal CTDA research tool that is submerged in the water to measure conductivity (salinity), temperature, and depth. cast and conventional CTDA research tool that is submerged in the water to measure conductivity (salinity), temperature, and depth. cast of the cruise. Wondering how they are different? I will tell you all about this 'shakedown' sampling in tomorrow's post. For now, please enjoy the first of what I hope is many wildlife sighting updates.

    First Wildlife Sighting

    During my time on the bridge this morning, I saw a Cape Petrel (Daption capense). This marks my first wildlife sighting in the Drake PassageStrait, connecting the Atlantic and Pacific oceans between Tierra del Fuego and the South Shetland Islands. Located about 100 mi (160 km) north of the Antarctic Peninsula, it is 600 mi (1,000 km) wide.. According to James Lowen in his Antarctic Wildlife: A Visitor's Guide (2011), these small petrels have a length of 14-16 inches (35-41cm) and a wingspan of 31-36 inches (81-91cm). These petrels have a beautiful dark brown-and-white pattern on their backs and upper wings in addition to a white rump patch with dark spots. I have seen another type of petrel during my time in the waters off the coast of New England. This species is known as the Wilson's Storm Petrel and differs in size (generally smaller) and usually have an all dark coloration except for a white rump patch.

    Cape Petrel
    This cape petrel (*Daption capense*) flies just above the surface of the water along side the Nathaniel B. Palmer. Between the rough seas and the speed of the birds, it was difficult to take a clear photo.

    Petrels are known to spend most of their lives at sea, only coming ashore for a short nesting period. Petrels have tube-shaped nostrils that vary in size with each species. These tubes help to drain the excess salt secreted from a duct near the bird's eye. The ability to remove excess salt allows these birds to spend longer times in saltwater without returning to land for drink freshwater. It was difficult to get a good picture of the Cape Petrel with the rough seas and ocean spray, but you can see more pictures on the Encyclopedia of Life website.

    Author
    Date
    Temperature
    Air Temperature: 30F/-1C, Water Temperature: 30F/-1C
    Wind Speed
    30-35 knots in the AM, dropped to 20-25 late in the day

    Comments

    Yi L. Block A

    Where does a Cape Petrel build its nest?

    HuiQian Z, Block A

    During the "Drake Passage", this type of petrels that survive in that condition, would it be possible for another type of petrels to survive there as well.

    Angela Y, Block F

    In what areas or climates do the Cape Petrel usually live in?

    Cara Pekarcik

    Angela - the Cape Petrels are common in the Drake Passage and along the coastlines of Antarctica.

    On 2016-09-13 19:44, PolarTREC wrote:

    Cara Pekarcik

    HuiQian - there are actually a number of different species of petrels that survive in these cold temperatures. In addition to the cape
    petrel, there are Atlantic petrels, grey petrels, blue petrels,
    soft-plumage petrels and others. There are also birds related to
    petrels like fulmars and albatross that also live in this cold
    environment.

    On 2016-09-13 18:32, PolarTREC wrote:

    Cara Pekarcik

    Hi Yi - great question! Cape petrels build there nest about 1km/.5 miles from shore. They usually build the nest of cliff or in between
    rocks. They use small rocks to make the nest and usually choose a spot
    that has an overhang for protection. One egg is produced each year and
    is incubated by both the male and female for 45 days.

    On 2016-09-13 16:18, PolarTREC wrote:

    Aili Aifan Block G

    Is that different depth of water has different measure of nutrient or it doesn`t matter with depth of the water ?

    mia sapienza

    Hi Ms.Pekarcik! This information iS very interesting.I was wondering if the concentration levels of dissolved inorganic nutrients in ocean water affect the characteristics of the nutrients?

    Tengfei L, Block B

    What does the concentration levels of dissolved inorganic nutrients shows about when it's at different level? Is it the more the better?

    Michael G Block F

    What is the Cape Petrel`s main source of food?

    Ningyu C, Block E

    How long is petrel's nesting period?

    Vivian Tran

    The journal mentioned that the diatoms have an outer shell called silica frustule. Is this outer shell similar to a regular animal or plant's cell wall?

    Winnie C Block F

    As you were monitoring the conditions in the water through the Drake passage, did you notice any changes?

    SheilaB Block E

    How long does FIA nutrient sampling usually take?

    Jasmine C., Block F

    Hi Ms. Pekarcik, On average, about how long does it normally take until the results of the FIA are available?

    Morgan Murphy

    What exactly are nisken bottles? Also do petrels eat fish?

    Cara Pekarcik

    Hi Mia - I am glad that you are so interested! Can you be more specific about your question. I don't understand what you are asking.
    Just hit the reply button below this comment. Thanks!

    On 2016-09-14 10:31, PolarTREC wrote:

    Melissa Z., Block A

    Hello Ms. Pekarsik,What were some of the chemical reactions produced by the FIA?

    Melissa Z., Block A

    Hello Ms. Pekarsik,What were some of the chemical reactions produced by the FIA?

    Melissa Z., Block A

    Hello Ms. Pekarsik,What were some of the chemical reactions produced by the FIA?

    Melissa Z., Block A

    Hello Ms. Pekarsik,What were some of the chemical reactions produced by the FIA?

    Cara Pekarcik

    Hi Aili - this question is a great one - and one that many scientist are still studying. Nutrient levels can change at depth, at different
    temperatures, at different distances from land, etc. By collecting
    water samples in different locations, scientists can compare the
    nutrient levels to be able to answer these and many other questions.

    On 2016-09-12 12:08, PolarTREC wrote:

    Cara Pekarcik

    Hi Morgan - take a look at some of the other journals - especially the one title The First Cast - you will learn everything about Nisken
    bottles. Petrels usually eat krill (a tiny, shrimp-like crustacean).

    On 2016-09-14 13:53, PolarTREC wrote:

    Cara Pekarcik

    Hi Michael! Cape petrels and many other petrel species each a diet largely containing crustaceans. Their favorite crustacean is krill,
    which can be found in abundance in the Southern Ocean.

    On 2016-09-12 13:47, PolarTREC wrote:

    Julia Brady Block G

    What is the Drake Passage?

    Cara Pekarcik

    Hi Ningyu! The answer to this question is probably a little different for each species of petrel (there are quite a few). Many only spend a
    few weeks on land to nest.

    On 2016-09-12 14:29, PolarTREC wrote:

    Jessica A, Block F

    How long can the birds stay in saltwater?

    Cara Pekarcik

    Great question Vivian! We are definitely going to be talking about plant cell walls when I return to NQ in October. The silica frustule is
    similar. I am not going to go into too much detail here because I am
    working on a journal about diatoms and will cover most of this
    information. I hope to have it ready in a few hours, so check back for
    today's journal later today or tomorrow.

    On 2016-09-12 14:43, PolarTREC wrote:

    Cara Pekarcik

    There are updates on the wind and air temperature posted on the ship's information board, so anyone can see the weather changes. When we first
    entered the Drake Passage, the sea conditions were a little rough. The
    wind speed increased over the next few days and increased the sea quite
    a bit.

    On 2016-09-12 15:39, PolarTREC wrote:

    Cara Pekarcik

    Julia - Take a look at the 9-9-16 journal called 'Welcome to the Drake' to find out more

    On 2016-09-14 16:20, PolarTREC wrote:

    Cara Pekarcik

    Hi Jasmine - I just spoke to Will Abbott and he said that it generally takes about 2 minutes per sample. Most of that time is required to get
    the sample through all of the tubing that you can see on the photos of
    the FIA. It only takes about 30 seconds for the sample to run through
    all of the reagents. Will collects water samples every 30 minutes and
    generally runs the FIA once he has at least 18 samples. I can tell you
    that Will is staying busy with all of the water samples he collects each
    day.

    On 2016-09-12 16:18, PolarTREC wrote:

    Cara Pekarcik

    Hi Jessica - if you are talking about the petrels, they are able to stay out in the open ocean all of their lives. Those tube noses allow
    them to drink salt water and remove the salt. No need to go ashore.
    They do head to shore for a few weeks to nest, but it is not necessary
    for survival, just reproduction.

    On 2016-09-13 04:23, PolarTREC wrote:

    Cara Pekarcik

    Hi Melissa - I just spoke with Will Abbott and he took me through the process required for the nitrate/nitrate nutrient analysis. It is a
    little over my non-chemist head, but I will let you know what I
    understand about the process. The seawater sample passes through a
    buffer to maintain a specific pH of the sample. This sample then reacts
    with sulfanilamide to produce a vibrant pink color. The FIA then reads
    the color intensity to determine the combination of nitrates and
    nitrites in the sample. The sample is then passed through a cadmium
    column to reduce all of the nitrate to nitrite. After this reaction,
    the color intensity is again measured to identify the amount of nitrite
    in the sample. The total nitrate is subtracted from the total nitrate +
    nitrite to calculate the total nitrate. I am pretty sure there are some
    other reactants in this process, but this is the general overview.

    On 2016-09-14 14:13, PolarTREC wrote:

    Cara Pekarcik

    Hi Tengfei - this is a really great question and one that I am not 100% equipped to answer yet. Once I spend more time with the chemical
    oceanographers I will have a better idea of how I can answer this
    question. I do know that different levels of dissolved nutrients can be
    caused by organisms using the nutrients or from location related to
    land. There are many factors that can affect the levels. In general,
    the scientists are looking at the levels in order to help understand
    which factors are important for diatoms in the Southern Ocean.

    More is not always better. There is probably different levels that are
    necessary for different organisms.

    On 2016-09-12 13:18, PolarTREC wrote:

    Loretta C. Block F

    How many samples do you collect?

    Cara Pekarcik

    Actually running the FIA only take a few seconds per sample.  By the end of the trip, Will had collected hundreds of samples.  The most time consuming part is making all of the reagents and trouble shooting problems.

    From: PolarTREC
    To:
    Sent: 10/20/2016 6:50 AM
    Subject: Re: SheilaB Block E commented on 10 September 2016 Nutrient Sampling

    ((

    Celia White Block F

    How quickly do you have to work to keep the diatoms alive?

    Iris Xie Block A

    Hi Ms. Pekarcik,At this point, have there been any outliers in your collection of data? What variable in your data tells where diatoms would most likely exist? What are the preferable conditions when collecting samples?

    Cara Pekarcik

    The speed at which we work is more related to keeping the temperature constant in order to keep the diatoms alive. If the diatoms have the
    conditions that they need to survive, then we have a higher chance of
    successfully collecting them.

    On 2016-09-15 10:23, PolarTREC wrote:

    Cara Pekarcik

    Will Abbott collects sample all day long at a 30 minute interval. He has a lot of samples each day!

    On 2016-09-13 10:27, PolarTREC wrote:

    Kevin C, Block B

    If the computer does determine that there are the concentrations of the desired nutrients, what does the desired nutrients do for the data?

    Cara Pekarcik

    Hi Iris - I can't really comment on this because we don't really have any data related to the diatoms yet - we are still incubating our first
    set of samples. There will be a lot of data by the end of the cruise,
    but analysis and other data collection and biological sampling will go
    on for many months after the cruise.

    On 2016-09-13 11:49, PolarTREC wrote:

    Cara Pekarcik

    Hi Kevin - there isn't a desired nutrient level, the machine is just testing to see how much of each nutrient is available in the water. If
    you read today's journal (9/13) you will see that diatoms require silica
    to make their outer frustules. By sampling for silica, the scientists
    can find waters that may be favorable for diatoms.

    On 2016-09-13 12:37, PolarTREC wrote: