DOC Characterization via Mass Spectrometry

Toolik Field Station, North Slope, AK

June 26, 2019

Video of the Day:

A small group of caribou woke me up from a nap on the tundra just north of the Brooks Range. Amazing animals to observe at such close range!

As a preface to this post, you’ll benefit from reading my previous entry on dissolved organic carbon (DOC). The focus of this entry is on one of the main methods used to characterize the organic components of DOC, mass spectrometry.

Mass Spectrometry

First, a bit on how this technique works. First, a given sample is bombarded with electrons, and its components are ionized. These ions are then accelerated into an electric or magnetic field, in which they undergo deflection. In the field, ions with the same ratio of mass to charge are deflected by the same amount. The separated ions are then detected, and results are displayed on a spectrum depicting intensity of signal (for detected ions) vs mass-to-charge ratio.1 2

Mass Spectrometer
The inner workings of a mass spectrometer. Image Sourge: Michigan State University.

Each bar on the x-axis of a mass spectrum represents an ion with its own mass-to-charge ratio (m/z), and the height of the bar on the y-axis indicates the relative amount of that ion present in the sample.2

Propane Mass Spectrum
Mass spectrum of propane and corresponding ion fragments. Image Source: Michigan State University.

If you use a mass spectrometer with sufficiently high resolution, you can obtain the molecular weight, hydrogen/carbon ratio, and oxygen/carbon ratio for compounds in a mixture.1 2 Molecular weight, hydrogen/carbon (H/C) ratio, and oxygen-carbon (O/C) ratio can be used to determine compound class. Van Krevelen diagrams, which plot H/C ratio as a function of O/C ratio, are also very useful in graphically depicting the different components in a mixture.

Characterizing Organic Matter
High resolution mass spectrometry allows for the determination of molecular weight, hydrogen/carbon (H/C) ratio, and oxygen/carbon (O/C) ratio. These data can be used to differentiate the different types of organic molecules in a mixture. Image Source: Dr. Collin Ward (Woods Hole Oceanographic Institute).

Van Krevelen Diagram
A Van Krevelen Diagram is a popular way of expressing high resolution mass spectrometry data. Each data point represents a cross-plot of the hydrogen/carbon (H/C) and oxygen/carbon (O/C) ratios for a given compound. Image Source: Dr. Collin Ward (Woods Hole Oceanographic Institute).

Aliphatic vs. Aromatic
Van Krevelen diagrams can be used to categorize compounds as aromatic or aliphatic, based on their H/C vs. O/C ratios. Image Source: Dr. Collin Ward (Woods Hole Oceanographic Institute).

DOC Characterization

Dr. Rose Cory’s lab has characterized stream DOC from the Arctic using high resolution mass spectrometry. Results suggest that DOC is a very complex mixture of organics, consisting of lignins, tannins, condensed aromatics, lipids, proteins, and carbohydrates.

Van Krevelen Diagram of DOC
Van Krevelen diagram of dissolved organic carbon from an Arctic stream. Compound classes are highlighted. Image Source: Dr. Rose Cory (University of Michigan).

But even high resolution mass spectrometry is limited in terms of what it can tell you about the components of a given mixture, especially if that mixture is complex. As the below graphic illustrates, it is very frequently the case that multiple molecules have identical molecular weights, H/C ratios, and O/C ratios. Accordingly, while we know about the general compound classes that are present in DOC, we have yet to shed much light on the specific identities of the chemical components.

Complexity of DOC
Dissolved organic carbon is quite the complex mixture. High resolution mass spectrometry allows for the determination of the mass, hydrogen/carbon (H/C) ratio, and oxygen/carbon (O/C) ratio of individual molecules. As the above figure illustrates, however, multiple complex molecules can have identical mass, H/C, and O/C. Image Source: Dr. Rose Cory (University of Michigan).

Comment below!

  1. Sparkman, O. David. Mass Spectrometry Desk Reference. Global View Publ., 2007. ↩︎ ↩︎

  2. Mass Spectrometry. Michigan State University.…;↩︎ ↩︎ ↩︎